Hilversum, 01-10-2019 – Scientific Volume Imaging (SVI) is thrilled to announce a collaboration with GATTAquant on the open GATTAscope project and introduce the HUYGENS Localizer as the recommended SMLM processing package.
HUYGENS Localizer introduces a seamless workflow to process, visualize and analyze Single Molecule Localization Microscopy (SMLM) data and facilitates a complete, fast, easy and very affordable super-resolution microscopy solution.
About the GATTAscope project
Jürgen Schmied, CEO at GATTAquant, elaborates on the project and it’s commercial partners: “Thanks to the GATTAscope project, any lab around the world can now easily set-up an affordable super-resolution microscope using the freely available GATTAscope design blue-prints and online tutorials. The recommended hardware parts can be purchased via the commercial partners. However, up until now we did not have a partner to take on the software (processing) part for the GATTAscope. The software is a very important part of accurate SMLM super-resolution microscopy, so we are very excited to have SVI-Huygens join as a commercial partner in our GATTAscope project. The HUYGENS Localizer package processes the acquired data extremely efficiently, and the results are truly impressive.”
About the HUYGENS Localizer
The HUYGENS Localizer is a new user-friendly and ultra fast super-resolution SMLM image processing package. Remko Dijkstra, Product Manager Super-resolution at SVI, highlights: “The HUYGENS Localizer is tailored for processing SMLM data highly efficiently and accurately. All kind of users, including beginners, can now easily process, visualize and analyze their STORM, PALM, GSD and/or (DNA)PAINT data. The processing-pipeline in the HUYGENS Localizer is GPU accelerated, enabling a super efficient and interactive user experience. The ease-of-use in combination with accuracy and high processing speed makes the Localizer a perfect match for the GATTAscope project. Using GATTquant’s 20nm GATTA-PAINT nanorulers, imaged on a GATTAscope, we were able to demonstrate that 20nm two-point resolution is reached with ease using the HUYGENS Localizer package.”
Super-resolution image demonstrating 20nm two-point resolution using GATTAquant 2x20nm DNA-PAINT nanorulers (GATTA-PAINT HiRes 20R) acquired with GATTAscope system. All 12.000 time-frames were processed with the HUYGENS Localizer in 9 seconds using an NVIDIA Quadro RTX5000 GPU. Top shows background corrected widefield equivalent (grayscale) overlayed with the HUYGENS Localizer super-resolution results (green). Inset: intensity profile of a single nanoruler shows two-point resolution of at least 20nm. Imaged by: GATTAquant GmbH, processed by Scientific Volume Imaging b.v.
Localization rendering: 5nm sampling, Guassian width based on uncertainty: normalized (40nm), intensity weighted.
More on GATTAquant GATTA-PAINT nanorulers: http://www.gattaquant.com/products/localization-based/gatta-paint-nanoruler.html