Which Components Do I Need?

Here you will find a list of important components to build up your own TIRF setup with four laser lines: from filters to camera to test samples and more. Please be aware that the following content does not claim to be complete and does not automatically fulfil all the needs you require from your system but just gives a brief overview about important parts. Also remember that not only the components itself but also the interplay between them has strong influence on the performance of your microscope.

Even though you do not plan to build your own system this list gives you a good overview about the complexity of an optical system. Please be aware that the displayed components and manufacturers are only suggestions and also alternative components or manufacturers can do a similar job.

Microscope Body

ComponentSpecificationManufacturer
BodyIX73P1F 1 Port BodyOlympus
RevolverIX3-D6RES RevolverOlympus
Filter CassetteIX3-RFACS-1-2 Filter CassetteOlympus
Illumination ArmIX3-ILL Illumination ArmOlympus
CondensorIX2-LWUCD CondensorOlympus
Filter45-LBD-IF Daylight Filter IFOlympus
Diffusor45FR Diffusor für IX3-ILLOlympus
LampU-LH100L-3-7 Hal.-Lamp Body
U-RMT-1-5 Connection Cable
JC12V100W Halogen Lamp
TH4-200-1-5 Power supply 230V
UYCP Connection Cable
Olympus
ObjectiveUPLSAPO100XO/1.4 ObjectivOlympus
Binocular TubusU-BI90-1-2 Binocular TubusOlympus
OcularWHN10X-1-7 Ocular
WHN10X-H-1-7 Ocular
Olympus
PlateU-OCMCROSSOlympus
AdapterU-TV1X-2-7 Adapter RingOlympus
C-MountU-CMAD3-1-7 C-Mount AdapterOlympus

Stage

ComponentSpecificationManufacturer
StagePILine XY stage, U-760.DNOSPI
Piezo StagePInano XYZ, P-545.3R8SPI
Adapter PlateM-545.SHPPI
InsetP-545.SH3 for PInanoPI

Laser

ComponentSpecificationManufacturer
Laser 642iBeam smart 640-S Diodenlaser, 640 nm, 150 mWToptica
Laser 488iBeam smart -PT-488HP Diodenlaser, 488 nm, 100 mWToptica

Camera

ComponentSpecificationManufacturer
CamerasCMOS, ORCA-Flash 4.0 LT, C11440, 4 MpxHamamatsu

Filter

ComponentSpecificationManufacturer
Filtercube 642ZET635/20x
ET655lp
ZT640rdc-UF1
in Olympus BX3
Chroma
Filtercube 561ZET561/10x
ET600/50m
ZT561rdc-UF1
in Olympus BX3
Chroma
Filtercube 488ZET488/10x
ET525/50m
ZT488rdc-UF1
in Olympus BX3
Chroma
DichroidZT594rdcChroma
DichroidZT542rdcChroma
DichroidZT514rdcChroma

Table

ComponentSpecificationManufacturer
Active Vibration Isolation Systemhalcyonics_i4, 400x500x90 mm3, M6/25Accurion

Optomechanics

ComponentSpecificationAmountManufacturer
Fiber PortPAFA-X-4-A, FC/PC, FC/APC2Thorlabs
Fiber Port MountCP08FP/M2Thorlabs
Mount + Mirror1”, KM100-E026Thorlabs
Posts½”, TR50/M11Thorlabs
Univ. Post HolderUPH40/M11Thorlabs
Optic Mount DichroidKM100C3Thorlabs
Cage Rod18”, ER184Thorlabs
Cage PlateCP02/M, Cage Stabilizer2Thorlabs
Lens TubeSM1L30, 80mm1Thorlabs
Lens TubeSM1L25, 66mm2Thorlabs
Cage Mounting BracketCP02B2Thorlabs
RailsXE25L225/M4Thorlabs
HardboardTB4, 3x 61 cm21Thorlabs
Aluminium TableMB4560/M, M61Thorlabs
Lens Mounting Plate35 mm, G0610470001QIOPTIQ
Lensf=100 mm, D=35 mm, mounted, G0632010001QIOPTIQ
Lens Mounting Platexy Translation, 25 mm, G0610250001QIOPTIQ
Lensf=25 mm, D=25 mm, mounted1QIOPTIQ
Cage MirrorElliptical, G0637130001QIOPTIQ
Linear StagesQ-545 Q-Motion2PI

Test Samples

ComponentSpecificationManufacturer
WidefieldGATTA-Confocal 350R and 270BGATTAquant
Localization-based SRGATTA-PAINT 80RGGATTAquant
Localization-based SRGATTA-PAINT HiRes 40RGATTAquant
Localization-based SRGATTA-PAINT HiRes 20RGATTAquant