How to build a TIRF microscope

Here you will find our video tutorials.


The introductory video explains the basic principles of the GATTAscope project.


The Overview video gives a first insight into the needed components to build a custom TIRF microscope and how the website can support you.

The Setup

After we presented the single components of the GATTAscope in the last video, we want to show you how all these components are positioned and guide you from the light source to the camera.

The Beam Path

In this video we show the beam path of the laser and why at least two mirrors are mandatory to align a beam.

The Cage System

The cage system is the essential optical component to adjust the focus and the TIRF angle.